Reporting LDL cholesterol results by clinical biochemistry laboratories in Czechia and Slovakia to improve the detection rate of familial hypercholesterolemia

Introduction This survey aims to assess the implementation of recommendations from the European Atherosclerosis Society (EAS) and the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) by clinical biochemistry laboratories in Czechia and Slovakia in their policies for reporting low-density lipoprotein cholesterol (LDL-C) concentrations. Materials and methods The web-based survey was distributed to all 383 Czech and Slovak clinical biochemistry laboratories that measure lipids by external quality assessment provider SEKK. A total of 17 single-answer questions were included. The questionnaire was focused on the detection and decision points in familial hypercholesterolemia (FH). All survey answers were taken into account. The laboratories followed the EFLM and EAS guidelines when they reported an interpretative comment considering FH diagnosis in adults. Results A total of 203 (53%) laboratories answered. Only 5% of laboratories added interpretative comments considering FH diagnosis when LDL-C concentrations are above 5.0 mmol/L in adults, and 3% of laboratories added interpretative comments considering FH diagnosis when LDL-C concentrations are above 4.0 mmol/L in children. Only 7% of laboratories reported goals for all cardiovascular risk categories (low, moderate, high, very high). Non-HDL cholesterol concentrations were calculated by 74% of responders. A significant number (51%) of participants did not measure apolipoprotein B, and 59% of laboratories did not measure lipoprotein(a). Conclusions Only a small portion of laboratories from Czechia and Slovakia reported high LDL-C results with interpretative comments considering FH diagnosis in adults, the laboratories did not follow the guidelines.


Introduction
Familial hypercholesterolemia (FH) is the most common monogenic disorder with autosomal dominant inheritance.The global prevalence rate of FH is approximately 1:300 (1).Children who have one parent with a heterozygous form have a 50% probability of having the disease.It is character-ized by high concentrations of low-density lipoprotein cholesterol (LDL-C) (2).This condition is clinically characterized by premature atherosclerotic cardiovascular disease (ASCVD).The basic lipid profile includes total cholesterol, triacylglycerols, LDL-C, and high-density lipoprotein cholester-ol (HDL-C).The typical laboratory feature of FH is serum LDL-C concentrations above 5 mmol/L in adult patients and LDL-C concentrations above 4 mmol/L in children.The disease's course can be asymptomatic; the first symptom may be a major cardiovascular event or sudden death.Current effective treatment of FH includes mainly non-pharmacological treatment (physical activity with a healthy diet), usually accompanied by pharmacotherapy that consists of statins, ezetimibe, PCSK-9 inhibitors, small interfering RNA molecules, and lipoprotein apheresis because, in most cases, nonpharmacological measures do not get LDL-C at a healthy level (3).The "Make Early Diagnosis and Prevent Early Death" (MED-PED) project is a worldwide activity to detect this condition.The Czech Republic is an active participant in this international venture, with a current FH detection rate of approximately 20% in the national register (4,5).Slovak colleagues also participate in the MED-PED project.Unfortunately, the majority of affected FH patients in the world are not diagnosed (6).Alert letters in patients with high LDL-C concentrations improve the FH detection rate (7).Clinical biochemistry professionals may improve the FH detection rate by adding interpretative comments to serum LDL-C concentrations above 5 mmol/L in adult patients and serum LDL cholesterol concentrations above 4 mmol/L in children.The comment "Consider heterozygous FH." should be provided in the laboratory report.This interpretative comment is recommended by both the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) and the European Atherosclerosis Society (EAS) (8).The evidence supporting LDL-C thresholds of 5 mmol/L in adults and 4 mmol/L in children is derived from LDL-C concentrations of first-degree relatives of genetically confirmed FH patients (9).The importance of FH detection was recognized at the international level, and the Prague declaration for FH screening across Europe was signed by European Union representatives.The screening approach is cost-effective (10).Implementation of the guidelines was not suggested to laboratories prior to this study.
The low FH detection rate is the primary reason we performed the questionnaire study, which aims to assess the implementation of recommendations from EAS and EFLM by clinical biochemistry laboratories in Czechia and Slovakia in their policies on reporting LDL-C concentrations (6).

Materials and methods
The web-based survey was distributed to all 383 Czech and Slovak clinical laboratories that measure lipids in external quality assessment (EQA) provided by SEKK (11).There were 31 laboratories from Slovakia and 172 laboratories from Czechia.Since Slovakia does not have its own EQA, Slovakia laboratories participate in Czechia cycles and follow similar policies.The study was performed in January 2023 in a regular EQA scheme.The questionnaire was created by three experts from the Czech Society for Atherosclerosis based on recommendations from EFLM and EAS.The most important questions focused on the detection and decision points in FH.Another three laboratory professionals from the EQA organization validated the content.The laboratories followed the EFLM and EAS guidelines when they reported an interpretative comment considering FH diagnosis in adults.
Laboratories received a notification e-mail before the survey.The survey was entered and distributed by the SEKK website to all participants.The answers were also entered electronically at the website, together with the lipid measurement results.Participation in the study was voluntary.No surveys were excluded.
All single-answer questions and possible responses are shown in Table 1.Laboratories were informed that their answers would be anonymously published.The survey was also intended to further educate laboratory professionals.Laboratories received recommendations after the completion of the study.

Statistical analysis
The Microsoft Office Excel program (Microsoft, Washington, USA) was used for data processing.The denominator for all percentage calculations was 203.The differences were assessed by Chisquare test using MedCalc 20.121 statistical soft-3 ware (MedCalc, Ostend, Belgium).The level of significance was defined as P < 0.05.

Results
A total of 203 laboratories participated in the study.The response rate was 53%, with 172 of the laboratories being from Czechia and 31 from Slovakia.Only 22 laboratories answered all questions.Only 5% of laboratories followed the guidelines by adding interpretative comments considering FH diagnosis when LDL-C concentrations are above 5.0 mmol/L in adults.Responses to the questions are displayed in Table 1.
A high portion of participants (51%) did not measure apolipoprotein B, and 59% of laboratories did not measure lipoprotein (a) (Lp(a)).
Czech laboratories reported more frequently non-HDL-C (P = 0.010) and more frequently measured Lp(a) in molar units (P = 0.030).Slovak laboratories reported more frequently lower LDL-C limit of ≤ 0.5 mmol/L (P = 0.012), upper LDL-C limit of 2.6 mmol/L (P = 0.001), and goals for all cardiovascular risk categories (P = 0.044).
The differences in answers between hospital and non-hospital laboratories are displayed in Table 2.

Question
Possible answers N (%)  The denominator for all percentage calculations was defined as the number of all participating laboratories (N = 203).LDL-C -low density lipoprotein cholesterol.LIS -laboratory information system.non-HDL-C -non high density lipoprotein cholesterol.

Discussion
We found that only a small portion of laboratories from Czechia and Slovakia reported high LDL-C results with interpretative comments that considered an FH diagnosis.was developed in molar units (28).In our study, 59% of laboratories that measure Lp(a) reported results in nmol/L units.
The recommendations were published in the national bulletin Fons to all laboratories after the survey was completed (29).
Laboratories were recommended to: The Czech Society for Clinical Biochemistry and the Czech Society for Atherosclerosis published a statement for diagnosing dyslipidaemias in 2017 (30).The poor implementation of current international EAS/EFLM recommendations may be caused by the fact that recommendations are in English language.Of course, the interpretation of the LDL-C results might be influenced by the presence of other diseases like diabetes, hypothyroidism, nephrotic syndrome, and some hepatic diseases.The first step in the diagnosis of primary hypercholesterolemia is the exclusion of secondary hypercholesterolemia.An interpretation comment could recommend glucose, thyroid-stimulating hormone, liver function tests, and proteinuria testing.
The limitations of this study are an incomplete response rate, as some laboratories did not answer all questions, and an inability to verify their answers.Data on LDL-C calculation are not concordant.A total of 85 participants provided information on the equation, but only 78 laboratories reported that they calculated LDL-C.Some respondents probably misunderstood the questions.
We conclude that the laboratories did not follow the guidelines, because only a small portion of laboratories from Czechia and Slovakia report high LDL cholesterol results with interpretative com-ments considering FH diagnosis in adults.More laboratories should consider apolipoprotein-B and Lp(a) measurements to refine the estimation of cardiovascular risk.We believe in the educational benefits of both the questionnaire used and this article.
Report interpretative comments considering FH diagnosis in adult patients with LDL-C concentrations above five mmol/L and children with LDL-C concentrations above four mmol/L.• Provide information on target LDL-C concentrations for all cardiovascular risk categories in the laboratory report.• Provide the upper decision threshold value in the laboratory report for the population at low risk (2.6 mmol/L), and the lower decision threshold value in the laboratory report should be the goal for the population at highest risk (0.5 mmol/L).• Not to use the Friedewald equation when plasma triacylglycerol concentrations exceed 4.5 mmol/L.• Use the Martin-Hopkins equation in patients with LDL-C concentrations below 1.8 mmol/L, in triacylglycerol concentration ranges of 2.0 -4.5 mmol/L, and in non-fasting samples.• Calculate non-HDL cholesterol concentrations.• Start measuring apolipoprotein B • Start measuring Lp(a) in molar units.